Brandon Hart -
Faculty Mentor(s):Dr. Vibhudutta Awasthi
Presentation Title: FOXO Expression in the Ileal Tissue of Rats Suffering from Hemorrhagic Shock
Forkhead box O-class (FOXO) proteins are a family of transcription factors that play important roles in regulating the expression of genes that mediate oxidative stress, heat shock, xenobiotic responses, innate immunity, metabolism, and autophagy. In this study we investigated the expression of FOXO transcription factors in ileum tissue of rats with hemorrhagic shock. Hemorrhagic shock was induced by withdrawing 45% of blood via an indwelling femoral artery catheter. The rats were euthanized at 1hr, 3hr, 6hr, and 24 hr to collect ileal samples. RNA from the tissue samples was isolated using Trizol reagent. The RNA (2 ug) was used to synthesize cDNA. Quantitative RT-PCR was performed to amplify the cDNA with rat FOXO1, FOXO3, FOXO4, and FOXO6 primers. The results showed that FOXO1 and FOXO6 expression was unchanged among various samples. FOXO3 showed a cyclical pattern where the expression was significantly reduced in early (1-3 hr) and 24 hr samples, but was close to the control levels in 6 hr samples. FOXO4, on the other hand, showed a significant (2-fold) increase in only 6 hr samples. Although, these preliminary results at transcription levels suggest a role of FOXO3 and/or FOXO4 in hemorrhagic shock, the observations have to be confirmed by assessing the expression of FOXO proteins by immunoblotting and activity assays.